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== Details ==

Agarose, acrylamide(polyacrylamide gel, SDS-polyacrylamide gel),Buffer(TBF) are requirement.

Buffer provides ions in the solution to ensure electricity conductivity power supply gel chamber,cellulose acetate.

Remember that DNA is the organic molecule, and is negatively charged. When electric field is applied to it, particles migrate towards positive electrode. An agrose gel is used to slow the movement of DNA and separate it by size.

How fast DNA will migrate depends upon the strength of electric field, buffer, density of agarose gel and size of the DNA.Smaller the size of DNA, faster it will move.Within an agarose gel, linear DNA migrate inversely proportional to the log10 of their molecular weight.