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== How to do PCR? ==

1.DNA is heated to separate th strandsof double helix at 95 degree celsius for one minute.

2.Nucleotides are added to PCR reaction,mix along with short DNA pieces of length 15-20 nucleotide called as primers.

3.The primers match the original DNA sequence which is to be amplified.

4.Heat stable DNA polymerase(Taq.DNA polymerase) is added adn mix is subjected to several cycle of replication in which new DNA strands are made by adding nucleotide to the primers.

5.DNA helices made of each cycle are separated by heating to serve as template from making more new DNA.

6.Billions of copies of original DNA can be generated in few hours.