Dispersion optimization to enhance PHB production in fed-batch cultures of Ralstonia eutropha.

Patnaik, P R (2006) Dispersion optimization to enhance PHB production in fed-batch cultures of Ralstonia eutropha. Bioresource technology, 97 (16). pp. 1994-2001. ISSN 0960-8524

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Abstract

Despite its many useful properties, microbial production of poly-beta-hydroxybutyrate (PHB) is not yet commercially competitive with synthetic polymers. One reason is inadequate optimization of the fermentation under industrial conditions. In this study, a physiologically reasonable and experimentally validated kinetic model for PHB synthesis by Ralstonia eutropha was incorporated into a dispersion model to simulate a large fed-batch bioreactor. Solutions of the model indicated that cell growth and PHB synthesis were maximum at Peclet numbers (Pe) between 20 and 30, representing limited finite dispersion. At these Peclet numbers, the optimum feed rates also showed lower consumptions of the substrates than at Pe=0. Since complete dispersion was also difficult to achieve in production-scale bioreactors, these results pointed to the possibility of exploiting controlled dispersion for productivity enhancement.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords:	PHB; Ralstonia eutropha; Fed-batch fermentation; Incomplete dispersion
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	17 Feb 2012 16:43
Last Modified:	17 Feb 2012 16:43
URI:	http://crdd.osdd.net/open/id/eprint/1027

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