A simple microassay for computing the hemolytic potency of drugs.

Raghava, G.P.S. and Goel, A and Singh, A M and Varshney, Grish C (1994) A simple microassay for computing the hemolytic potency of drugs. BioTechniques, 17 (6). pp. 1148-53. ISSN 0736-6205

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A simple microassay and computer program are described for determining the erythrocyte hemolytic potency of drugs in vitro. This microassay is sensitive for both micro as well as macro ranges of hemoglobin concentration. An ELISA reader has been adapted to read erythrocyte lysis (hemolysis), which reduces the number and culture of replicates. A computer program was developed that calculates parameters such as C50 (concentration of drug causing 50% hemolysis), C100 (concentration of drug causing 100% hemolysis) and beta (slope of the curve) and graphically expresses the hemolytic patterns of various drugs simultaneously. The program can obtain optical densities directly from a 96-well plate ELISA reader by interfacing the microplate reader to the computer or by using a keyboard. This method is useful for screening a large number of hemolytic drugs and requires lower amounts of test compounds. It may also be applicable to quantitative functional assays, such as complement-mediated hemolysis and enumeration of antibody-secreting cells. The program can be obtained from the authors on request.

Item Type: Article
Additional Information: Copyright of this article belongs to Informa Healthcare.
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 09 Jan 2012 07:33
Last Modified: 07 Jan 2015 05:48
URI: http://crdd.osdd.net/open/id/eprint/109

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