Indibacter alkaliphilus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake.

Anil Kumar, P and Srinivas, T N R and Madhu, S and Manorama, R and Shivaji, S (2010) Indibacter alkaliphilus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake. International journal of systematic and evolutionary microbiology, 60 (Pt 4). pp. 721-6. ISSN 1466-5026

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A novel Gram-stain-negative, rod-shaped, non-motile bacterium, strain LW1(T), was isolated from a water sample collected at a depth of 3.5 m from Lonar Lake, Buldhana district, Maharashtra, India. The cell suspension was reddish-orange due to the presence of carotenoids. Strain LW1(T) was positive for catalase, oxidase, ornithine decarboxylase and lysine decarboxylase and negative for gelatinase, urease and lipase. Fatty acids were dominated by branched-chain fatty acids (>76 %), with a high abundance of iso-C(15 : 0) (48 %), anteiso-C(15 : 0) (7 %) and iso-C(17 : 0) 3-OH (11 %). Strain LW1(T) contained MK-4 and MK-7 as the major respiratory quinones and phosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine as the major phospholipids. A blast sequence similarity search based on 16S rRNA gene sequences indicated that members of the genera Belliella and Aquiflexum were the nearest phylogenetic neighbours with similarities of 91.8-92.3 %. Phylogenetic analyses indicated that strain LW1(T) formed a deep-rooted lineage distinct from the clades represented by the genera Belliella, Aquiflexum, Cyclobacterium, Echinicola and Algoriphagus. Based on the above-mentioned phenotypic and phylogenetic characteristics, it is proposed that strain LW1(T) represents a novel species in a new genus, Indibacter alkaliphilus gen. nov., sp. nov. (type strain LW1(T)=KCTC 22604(T)=CCUG 57479(T)). The genomic DNA G+C content of strain LW1(T) is 42.7+/-1 mol%.

Item Type: Article
Additional Information: Copyright of this article belongs to SGM.
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 28 Feb 2012 16:09
Last Modified: 28 Feb 2012 16:09

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