Functional characterization of a novel Mn(2+) dependent protein serine/threonine kinase KpnK, produced by Klebsiella pneumoniae strain MGH78578.

Srinivasan, Vijaya Bharathi and Vaidyanathan, Vasanth and Mondal, Amitabha and Venkataramaiah, Manjunath and Rajamohan, Govindan (2012) Functional characterization of a novel Mn(2+) dependent protein serine/threonine kinase KpnK, produced by Klebsiella pneumoniae strain MGH78578. FEBS letters, 586 (21). pp. 3778-86. ISSN 1873-3468

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Abstract

Klebsiella pneumoniae MGH78578 contains ∼500 uncharacterized signaling proteins and in this study, we characterized the biological functions of a novel eukaryotic-like serine/threonine kinase; ESTK (KpnK). Studies demonstrated that KpnK undergoes autophosphorylation within the pH range 7.0-7.5 at 37°C in a time- and concentration- dependent manner, with Mn(2+) as its cofactor. The ΔkpnK mutant exhibited higher sensitivity to gastrointestinal and oxidative stresses. Deletion of kpnK resulted in a two to threefold increased susceptibility towards imipenem, cefepime, ceftriaxone and ceftazidime. Our study has provided overall evidence for the involvement of ESTK in regulating bacterial physiology, stress response and drug resistance. This report has unmasked the occurrence of Ser/Thr kinase mediated signaling for the first time in K. pneumoniae. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: KpnKphosphorylatesKpnK by protein kinase assay (View interaction).

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords:	Nosocomial pathogen; Antimicrobial resistance; Ser/Thr kinase;Klebsiella pneumoniae
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	31 Jan 2013 04:33
Last Modified:	31 Jan 2013 04:33
URI:	http://crdd.osdd.net/open/id/eprint/1219

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