Differential expression of NF-kappaB in mycobacteria infected THP-1 affects apoptosis.

Dhiman, Rohan and Raje, Manoj and Majumdar, Sekhar (2007) Differential expression of NF-kappaB in mycobacteria infected THP-1 affects apoptosis. Biochimica et biophysica acta, 1770 (4). pp. 649-58. ISSN 0006-3002

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Abstract

The present study was conducted to see the role of NF-kappaB in virulent (Mycobacterium tuberculosis H37Rv) and avirulent (M. tuberculosis H37Ra) mycobacterial infection in THP-1 cells. To inactivate NF-kappaB, pCMV-IkappaBalphaM dn containing THP-1 cell line was generated which showed marked increase in apoptosis with M. tuberculosis H37Rv and M. tuberculosis H37Ra. Infected THP-1-IkappaBalphaM dn cells showed decrease in mitochondrial membrane potential, cytochrome c release, activation of caspase-3 and enhanced TNF-alpha production. Increase in apoptosis of infected THP-1-IkappaBalphaM dn cells resulted in inhibition of intracellular mycobacterial growth. Differential NF-kappaB activation potential was observed with M. tuberculosis H37Rv and M. tuberculosis H37Ra. Both the strains activated NF-kappaB after 4 h in THP-1 cells however after 48 h only M. tuberculosis H37Rv activated NF-kappaB which lead to up-regulation of bcl-2 family anti-apoptotic member, bfl-1/A1. Our results indicated that NF-kappaB activation may be a determinant factor for the success of virulent mycobacteria within macrophages.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords:	THP-1 cells; Mycobacteria; NF-κB; Apoptosis; bfl-1/A1
Subjects:	Q Science > QD Chemistry
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	09 Jan 2012 05:06
Last Modified:	09 Jan 2012 05:06
URI:	http://crdd.osdd.net/open/id/eprint/125

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