Bacterial community structure of a pesticide-contaminated site and assessment of changes induced in community structure during bioremediation.

Paul, Debarati and Pandey, Gunjan and Meier, Christoph and van der Meer, Jan Roelof and Jain, R K (2006) Bacterial community structure of a pesticide-contaminated site and assessment of changes induced in community structure during bioremediation. FEMS microbiology ecology, 57 (1). pp. 116-27. ISSN 0168-6496

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Abstract

The introduction of culture-independent molecular screening techniques, especially based on 16S rRNA gene sequences, has allowed microbiologists to examine a facet of microbial diversity not necessarily reflected by the results of culturing studies. The bacterial community structure was studied for a pesticide-contaminated site that was subsequently remediated using an efficient degradative strain Arthrobacter protophormiae RKJ100. The efficiency of the bioremediation process was assessed by monitoring the depletion of the pollutant, and the effect of addition of an exogenous strain on the existing soil community structure was determined using molecular techniques. The 16S rRNA gene pool amplified from the soil metagenome was cloned and restriction fragment length polymorphism studies revealed 46 different phylotypes on the basis of similar banding patterns. Sequencing of representative clones of each phylotype showed that the community structure of the pesticide-contaminated soil was mainly constituted by Proteobacteria and Actinomycetes. Terminal restriction fragment length polymorphism analysis showed only nonsignificant changes in community structure during the process of bioremediation. Immobilized cells of strain RKJ100 enhanced pollutant degradation but seemed to have no detectable effects on the existing bacterial community structure.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Wiley.
Uncontrolled Keywords:	Bioremediation; T-RFLP; ARDRA; p-nitrophenol; community structure.
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	09 Jan 2012 04:22
Last Modified:	03 Apr 2012 07:20
URI:	http://crdd.osdd.net/open/id/eprint/144

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