Kocuria indica sp. nov., isolated from a sediment sample.

Dastager, Syed G and Tang, Shan-Kun and Srinivasan, Krishnamurthi and Lee, Jae-Chan and Li, Wen-Jun (2014) Kocuria indica sp. nov., isolated from a sediment sample. International journal of systematic and evolutionary microbiology, 64 (Pt 3). pp. 869-74. ISSN 1466-5034

PDF krishnamurti2014.1.pdf - Published Version Restricted to Registered users only Download (219Kb) | Request a copy

Abstract

An actinobacterial strain was isolated from a sediment sample from Chorao Island, in Goa province, India, and subjected to a taxonomic investigation. The isolate, designated NIO-1021(T), was a Gram-stain positive, aerobic, non-motile and coccoid. Strain NIO-1021(T) was identified as a member of the genus Kocuria by a polyphasic approach. Strain NIO-1021(T) could be differentiated from other members of the genus Kocuria on the basis of physiology and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence similarity of strain NIO-1021(T) to the most closely related species, Kocuria marina KCTC 9943(T), was 98.6% with 19 nt differences). Furthermore, DNA-DNA hybridization analysis revealed that the novel strain had lower relatedness with the type strains of other members of the genus Kocuria. The strain formed a monophyletic clade with K. marina with 100% bootstrap values. The major phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified lipids. The predominant menaquinone was MK-7(H2). The major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The DNA G+C content of strain NIO-1021(T) was 60.5 mol%. Chemotaxonomic and phylogenetic properties of the strain were consistent with its classification as representing a member of the genus Kocuria. On the basis of phenotypic, chemotypic and molecular characteristics, strain NIO-1021(T) is considered to represent a novel species of the genus Kocuria, for which the name Kocuria indica sp. nov. is proposed, with strain NIO-1021(T) ( = NCIM 5455(T) = DSM 25126(T) = CCTCC AA 209050(T)) as the type strain.

Item Type:	Article
Additional Information:	Copyright of this article belongs to International Union of Microbiological Societies.
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	29 May 2014 08:35
Last Modified:	29 May 2014 08:35
URI:	http://crdd.osdd.net/open/id/eprint/1494

Actions (login required)

View Item