Bajaj, Abhay and Mayilraj, Shanmugam and Mudiam, Mohana Krishna Reddy and Patel, Devendra Kumar and Manickam, Natesan (2014) Isolation and functional analysis of a glycolipid producing Rhodococcus sp. strain IITR03 with potential for degradation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT). Bioresource technology, 167. pp. 398-406. ISSN 1873-2976
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A 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degrading bacterium strain IITR03 producing trehalolipid was isolated and characterized from a pesticides contaminated soil. The strain IITR03 was identified as a member of the genus Rhodococcus based on polyphasic studies. Under aqueous culture conditions, the strain IITR03 degraded 282 μM of DDT and could also utilize 10mM concentration each of 4-chlorobenzoic acid, 3-chlorobenzoic acid and benzoic acid as sole carbon and energy source. The catechol 1,2-dioxygenase enzyme activity resulted in conversion of catechol to form cis,cis-muconic acid. Cloning and sequencing of partial nucleotide sequence of catechol 1,2-dioxygenase gene (cat) from strain IITR03 revealed its similarity to catA gene present in Rhodococcus sp. strain Lin-2 (97% identity) and Rhodococcus strain AN22 (96% identity) degrading benzoate and aniline, respectively. The results suggest that the strain IITR03 could be useful for field bioremediation studies of DDT-residues and chlorinated aromatic compounds present in contaminated sites.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Elsevier Publisher. |
| Uncontrolled Keywords: | Rhodococcus sp.; Biodegradation; Chloroaromatic compound; Biosurfactant |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 27 Jan 2015 05:05 |
| Last Modified: | 27 Jan 2015 05:05 |
| URI: | http://crdd.osdd.net/open/id/eprint/1524 |
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