Sabherwal, Priyanka and Shorie, Munish and Pathania, Preeti and Chaudhary, Shilpa and Bhasin, K K and Bhalla, Vijayender and Suri, C Raman (2014) Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene. Analytical chemistry, 86 (15). pp. 7200-4. ISSN 1520-6882
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Combining synthetic macromolecules and biomolecular recognition units are promising in developing novel diagnostic and analysis techniques for detecting environmental and/or clinically important substances. Fluorescence resonance energy transfer (FRET) apta-immunosensor for explosive detection is reported using 2,4,6-trinitrotoluene (TNT) specific aptamer and antibodies tagged with respective FRET pair dyes in a sandwich immunoassay format. FITC-labeled aptamer was used as a binder molecule in the newly developed apta-immunoassay format where the recognition element was specific anti-TNT antibody labeled with rhodamine isothiocyanate. The newly developed sensing platform showed excellent sensitivity with a detection limit of the order of 0.4 nM presenting a promising candidate for routine screening of TNT in samples.
| Item Type: | Article |
|---|---|
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 09 Feb 2015 04:21 |
| Last Modified: | 09 Feb 2015 04:21 |
| URI: | http://crdd.osdd.net/open/id/eprint/1538 |
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