Kaur, Gurmeet and Subramanian, Srikrishna (2014) Repurposing TRASH: emergence of the enzyme organomercurial lyase from a non-catalytic zinc finger scaffold. Journal of structural biology, 188 (1). pp. 16-21. ISSN 1095-8657
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The mercury resistance pathway enzyme organomercurial lyase (MerB) catalyzes the conversion of organomercurials to ionic mercury (Hg(2+)). Here, we provide evidence for the emergence of this enzyme from a TRASH-like, non-enzymatic, treble-clef zinc finger ancestor by domain duplication and fusion. Surprisingly, the structure-stabilizing metal-binding core of the treble-clef appears to have been repurposed in evolution to serve a catalytic role. Novel enzymatic functions are believed to have evolved from ancestral generalist catalytic scaffolds or from already specialized enzymes with catalytic promiscuity. The emergence of MerB from a zinc finger ancestor serves as a rare example of how a novel enzyme may emerge from a non-catalytic scaffold with a related binding function.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Domain duplication; Domain fusion; Enzyme evolution; MerA; MerH; NosL; Promiscuous enzymes |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 09 Feb 2015 10:51 |
| Last Modified: | 09 Feb 2015 10:51 |
| URI: | http://crdd.osdd.net/open/id/eprint/1561 |
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