Chopra, Adity and Tuteja, Satish and Sachdeva, Naresh and Bhasin, K K and Bhalla, Vijayender and Suri, C Raman (2013) CdTe nanobioprobe based optoelectrochemical immunodetection of diabetic marker HbA1c. Biosensors & bioelectronics, 44. pp. 132-5. ISSN 1873-4235
Full text not available from this repository. (Request a copy)Abstract
Highly luminescent water soluble CdTe quantum dots (QDs) were synthesized and conjugated with anti-HbA1c antibody to generate specific nanobioprobe. A sandwich immunoassay model was employed using capture HbA1c antibody as a specific receptor molecule and the QD-labeled secondary antibody as a dual (fluorescence cum electrochemical) tracer to quantify the concentration of HbA1c. A linear increase in current was observed for HbA1c analytical standards with a R(2) value of 0.990 and coefficient of variance ~5%. A comparison between HPLC and dual immunoassay for clinical samples showed a correlation coefficient of 89% and 96% for fluorescence and electrochemical detection methods respectively. The QD-based immunoassay shows great promise for rapid reproducible and cost effective analysis of HbA1c in clinical samples.
| Item Type: | Article |
|---|---|
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 11 Feb 2015 07:14 |
| Last Modified: | 11 Feb 2015 07:14 |
| URI: | http://crdd.osdd.net/open/id/eprint/1615 |
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