Bilobed shape of PadA reveals the connectivity from single to multi-domain bacterial plasminogen activators.

Singh, Satish and Rathore, Yogendra Singh and Bhando, Timsy and Hade, Mangesh Dattu and Ganguly, Ashish and Dikshit, Kanak L (2015) Bilobed shape of PadA reveals the connectivity from single to multi-domain bacterial plasminogen activators. International journal of biological macromolecules, 78. pp. 370-8. ISSN 1879-0003

PDF KLD 2015 (In Press) singh2015.pdf - Accepted Version Restricted to Registered users only Download (1822Kb)

Abstract

The bacterial plasminogen activator, PadA activates bovine, ovine and caprine plasminogen but remains inert toward human plasminogen. It shows high sequence homology with human plasminogen activator, staphylokinase (SAK) but generates active-site in bovine plasminogen non-proteolytically, similar to streptokinase (SK). To examine the structural requirements for the function of this unique cofactor, attempts were made to visualize solution structure of the PadA using small-angle X-ray scattering (SAXS) data and compare its shape profile with structural models based on crystal structures of staphylokinase and streptokinase domains. The bilobal shape solved for the PadA matched closely with the structural model of α-domain of SK rather than its sequence homolog, SAK. The SAXS based solution structure of the PadA exhibited an extra volume and high mobility around Y(90)DKAEK(95) and P(104)ITES(108) loop regions that were found to play a crucial role in its cofactor function. Structure and sequence analysis of bacterial cofactors and mammalian plasminogens displayed evolutionary conservation of crucial complimentary amino acids required for making a functional binary activator complex between bacterial plasminogen activators and their cognate partner plasminogen. These studies highlighted the importance of structure-function related evolutionary strategies adopted by bacteria for exploiting mammalian plasminogen activation system and its understanding may help in designing and the development of new thrombolytic agents for clinical interventions.

Item Type:	Article
Additional Information:	copyright of this article belongs to Elsevier.
Uncontrolled Keywords:	PadA; Plasminogen; Small angle X-ray scattering; Staphylokinase; Streptokinase
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	13 Jul 2015 12:46
Last Modified:	14 Jul 2015 05:13
URI:	http://crdd.osdd.net/open/id/eprint/1637

Actions (login required)

View Item