Chopra, Adity and Rawat, Sanjay and Bhalla, Vijayender and Suri, C Raman (2014) Point-of-Care Amperometric Testing of Diabetic Marker (HbA1c) Using Specific Electroactive Antibodies. Electroanalysis, 26 (3). pp. 469-472. ISSN 10400397
Full text not available from this repository. (Request a copy)Abstract
Specific immune detection of glycated hemoglobin is still a great challenge owing to the small epitopic difference between Hemoglobin (Hb) and HbA1c. We report a new electrochemical immunoassay format for point of care testing of HbA1c. A conducting self-assembled monolayer of mercaptophenyl boronic acid (MPBA) was used as a capture layer for binding of glycated proteins and ferrocene tagged anti-HbA1c antibody (FcAb) as a tracer molecule on a gold screen printed electrode. Validation of the new HbA1c assay was carried out using 6 clinical samples with known HPLC values and a correlation coefficient of 98 % was observed.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Wiley. |
| Uncontrolled Keywords: | HbA1c;Ferrocene tagged antibody;Self-assembled monolayer;Electrochemical immunoassay |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 20 Jul 2015 03:54 |
| Last Modified: | 20 Jul 2015 03:54 |
| URI: | http://crdd.osdd.net/open/id/eprint/1706 |
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