Bacillus cellulasensis sp. nov., isolated from marine sediment.

Mawlankar, Rahul and Thorat, Meghana N and Krishnamurthi, Srinivasan and Dastager, Syed G (2016) Bacillus cellulasensis sp. nov., isolated from marine sediment. Archives of microbiology, 198 (1). pp. 83-9. ISSN 1432-072X

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Abstract

A novel bacterial strain NIO-1130(T) was isolated from sediment sample taken from Chorao Island, Goa Province, India, and subjected to a taxonomic investigation. The strain was Gram-positive, aerobic, and motile. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and strain NIO-1130(T) showed highest sequence similarity with Bacillus halosaccharovorans DSM 25387(T) (98.4 %) and Bacillus niabensis CIP 109816(T) (98.1 %), whereas other Bacillus species showed <97.0 % similarity. Tree based on gyrB gene sequence revealed that strain bacillus group. The major menaquinone was MK-7 and the predominant cellular fatty acids were iso-C15:0, anteiso-C15:0, iso-C17:0, and anteiso-C17:0. The strain showed a DNA G+C content of 39.9 mol%. DNA-DNA hybridization studies revealed that strain NIO-1130(T) exhibits 70 % similarity with Bacillus halosaccharovorans DSM 25387(T) and Bacillus niabensis CIP 109816(T). On the basis of physiological, biochemical, chemotaxonomic and phylogenetic analyses, we consider the isolate to represent a novel species of the genus Bacillus, for which the name Bacillus cellulasensis sp. nov., is proposed. The type strain is NIO-1130(T) (=NCIM 5461(T)=CCTCC AB 2011126(T)).

Item Type:	Article
Additional Information:	Copyright of this article belongs to Springer.
Uncontrolled Keywords:	Bacillus sp; Cellulase; Marine sediment; Polyphasic taxonomy
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	29 Jan 2016 04:40
Last Modified:	29 Sep 2016 07:35
URI:	http://crdd.osdd.net/open/id/eprint/1752

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