Rhodococcus lactis sp. nov., an actinobacterium isolated from sludge of a dairy waste treatment.

Singh, Pradip Kumar and Kumari, Annu and Chawla, Niharika and Pinnaka, Anil Kumar and Korpole, Suresh (2015) Rhodococcus lactis sp. nov., an actinobacterium isolated from sludge of a dairy waste treatment. International journal of systematic and evolutionary microbiology, 65. ISSN 1466-5034

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A Gram-positive, non-motile and aerobic bacterium designated as DW151BT was isolated from a sludge sample of a dairy industry effluent treatment plant. The 16S rRNA gene sequence of strain DW151BT assigned it to the genus Rhodococcus. It displayed significant similarity with validly published species like Rhodococcus pyridinivorans PDB9T (98.8%), R. gordoniae W 4937T (98.6%), R. rhodochrous DSM 43241T (98.5%) and R. artemisiae YIM 65754T (97.5%). However, strain DW151BT differed with the phylogenetically closely related species in various phenotypic properties. The cellular polar lipid composed of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) as major lipids, MK-8(H2) as major menaquinone and meso-diaminopimelic acid as cell-wall peptidoglycan. The fatty acid profile consisted mainly C16:0, C18:1cis9, C16:1cis9. Presence of C16:0 and diphosphatidylglycerol as major fatty acid and polar lipid, respectively, were in accordance with chemotaxonomic markers of the genus Rhodococcus. The G+C content of the strain is 69.9 mol% which is found to be within the limits reported for the members of this genus. Further, strain DW151BT showed low similarity at the whole genome level in DNA-DNA hybridization experiment with phylogenetically closely related strains. Considering the low similarity at the genome level and differences in phenotypic properties, we conclude that the strain DW151BT represent a novel species of the genus Rhodococcus, for which the name Rhodococcus lactis sp. nov. is proposed. The type strain of Rhodococcus lactis sp. nov. is DW151BT (=MTCC 12279T =DSM 45625T).

Item Type: Article
Additional Information: Copyright of this article belongs to Microbiology Society
Uncontrolled Keywords: maximum-likelihood; neighbour-joining
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 29 Jan 2016 04:33
Last Modified: 29 Jan 2016 04:33
URI: http://crdd.osdd.net/open/id/eprint/1755

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