Candida digboiensis sp. nov., a novel anamorphic yeast species from an acidic tar sludge-contaminated oilfield.

Prasad, G S and Mayilraj, Shanmugam and Sood, Nitu and Singh, Vijeyta and Biswas, Kakoli and Lal, Banwari (2005) Candida digboiensis sp. nov., a novel anamorphic yeast species from an acidic tar sludge-contaminated oilfield. International journal of systematic and evolutionary microbiology, 55 (Pt 2). pp. 967-72. ISSN 1466-5026

PDF prasad2005.pdf - Published Version Restricted to Registered users only Download (147Kb) | Request a copy

Abstract

Two strains (TERI-6(T) and TERI-7) of a novel yeast species were isolated from acidic tar sludge-contaminated soil samples collected from Digboi Refinery, Assam, India. These two yeast strains were morphologically, physiologically and phylogenetically identical to each other. No sexual reproduction was observed on corn meal, malt, Gorodkowa, YM or V8 agars. Physiologically, the novel isolates were most closely related to Candida blankii, but differed in eight physiological tests. The prominent differences were the ability of the isolates to assimilate melibiose and inulin and their inability to assimilate d-glucuronate, succinate and citrate. Phylogenetic analysis using the D1/D2 variable domain showed that the closest relative of these strains is C. blankii (2.8 % divergence). Other related species are Zygoascus hellenicus and Candida bituminiphila. The isolates differed from C. blankii by 11 base substitutions in the 18S rRNA gene sequence and by 58 base substitutions in the internal transcribed spacer sequences. The physiological, biochemical and molecular data support the contention that strains TERI-6(T) and TERI-7 represent a novel species, for which the name Candida digboiensis sp. nov. is proposed. The type strain is TERI-6(T) (=MTCC 4371(T)=CBS 9800(T)=JCM 12300(T)).

Item Type:	Article
Additional Information:	Copyright of this article belongs to International Union of Microbiological Societies.
Uncontrolled Keywords:	ITS, internal transcribed spacer SSU, small subunit
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	08 Jan 2012 05:42
Last Modified:	09 Jan 2015 08:37
URI:	http://crdd.osdd.net/open/id/eprint/183

Actions (login required)

View Item