Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth.

Haque, Farazul and Alfatah, Md and Ganesan, K and Bhattacharyya, Mani Shankar (2016) Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth. Scientific reports, 6. p. 23575. ISSN 2045-2322

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Abstract

Candida albicans causes superficial and life-threatening systemic infections. These are difficult to treat often due to drug resistance, particularly because C. albicans biofilms are inherently resistant to most antifungals. Sophorolipid (SL), a glycolipid biosurfactant, has been shown to have antimicrobial and anticancer properties. In this study, we investigated the effect of SL on C. albicans biofilm formation and preformed biofilms. SL was found to inhibit C. albicans biofilm formation as well as reduce the viability of preformed biofilms. Moreover, SL, when used along with amphotericin B (AmB) or fluconazole (FLZ), was found to act synergistically against biofilm formation and preformed biofilms. Effect of SL on C. albicans biofilm formation was further visualized by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), which revealed absence of hyphae, typical biofilm architecture and alteration in the morphology of biofilm cells. We also found that SL downregulates the expression of hypha specific genes HWP1, ALS1, ALS3, ECE1 and SAP4, which possibly explains the inhibitory effect of SL on hyphae and biofilm formation.

Item Type:	Article
Additional Information:	Open Access
Uncontrolled Keywords:	Biofilm; Drug Development
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	29 Sep 2016 10:32
Last Modified:	29 Sep 2016 10:32
URI:	http://crdd.osdd.net/open/id/eprint/1871

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