Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture.

Priyanka, Anu and Solanki, Vipul and Parkesh, Raman and Thakur, Krishan Gopal (2016) Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture. Proteins, 84 (10). pp. 1558-63. ISSN 1097-0134

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Abstract

Human SIRT7 is an NAD(+) dependent deacetylase, which belongs to sirtuin family of proteins. SIRT7, like other sirtuins has conserved catalytic domain and is flanked by N- and C-terminal domains reported to play vital functional roles. Here, we report the crystal structure of the N-terminal domain of human SIRT7 (SIRT7(NTD) ) at 2.3 Å resolution as MBP-SIRT7(NTD) fusion protein. SIRT7(NTD) adopts three-helical domain architecture and comparative structural analyses suggest similarities to some DNA binding motifs and transcription regulators. We also report here the importance of N- and C-terminal domains in soluble expression of SIRT7. Proteins 2016; 84:1558-1563. © 2016 Wiley Periodicals, Inc.

Item Type:	Article
Additional Information:	Open Access
Uncontrolled Keywords:	Human SIRT7; histone deacetylase; maltose binding protein; peptide mass fingerprinting; sirtuins; x-ray crystallography
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	29 Sep 2016 07:13
Last Modified:	29 Sep 2016 07:13
URI:	http://crdd.osdd.net/open/id/eprint/1897

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