Tuteja, Satish K and Chen, Rui and Kukkar, Manil and Song, Chung Kil and Mutreja, Ruchi and Singh, Suman and Paul, Ashok K and Lee, Haiwon and Kim, Ki-Hyun and Deep, Akash and Suri, C Raman (2016) A label-free electrochemical immunosensor for the detection of cardiac marker using graphene quantum dots (GQDs). Biosensors & bioelectronics, 86. pp. 548-56. ISSN 1873-4235

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S0956-...

Abstract

A label-free immunosensor based on electrochemical impedance spectroscopy has been developed for the sensitive detection of a cardiac biomarker myoglobin (cMyo). Hydrothermally synthesized graphene quantum dots (GQDs) have been used as an immobilized template on screen printed electrodes for the construction of an impedimetric sensor platform. The GQDs-modified electrode was conjugated with highly specific anti-myoglobin antibodies to develop the desired immunosensor. The values of charge transfer resistance (Rct) were monitored as a function of varying antigen concentration. The Rct value of the immunosensor showed a linear increase (from 0.20 to 0.31kΩ) in the range of 0.01-100ng/mL cMyo. The specific detection of cMyo was also made in the presence of other competing proteins. The limit of detection for the proposed immunosensor was estimated as 0.01ng/mL which is comparable to the standard ELISA techniques.

Item Type: Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords: raphene quantum dots; Myoglobin; Electrochemical Impedance Spectroscopy; Label-free immunosensor; Cardiac marker
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 29 Sep 2016 06:00
Last Modified: 29 Sep 2016 06:00
URI: http://crdd.osdd.net/open/id/eprint/1909

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