Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India.

Kaur, Simerpreet and Rana, Vibhuti and Singh, Pooja and Trivedi, Garima and Anand, Shashi and Kaur, Amanpreet and Gupta, Pawan and Jain, Amita and Sharma, Charu (2016) Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India. Tuberculosis (Edinburgh, Scotland), 96. pp. 96-101. ISSN 1873-281X

Full text not available from this repository. (Request a copy)
Official URL: http://www.sciencedirect.com/science/article/pii/S...

Abstract

Twenty-nine Kanamycin resistant clinical isolates of Mycobacterium tuberculosis from Northern India were screened to evaluate genetic mutations in rrs gene, eis gene with its promoter, and whiB7 gene along with its 5'UTR. 14 strains (~48.0%) collectively exhibited mutations in rrs, eis or whiB7 target regions. While the highest frequency of mutations was found in rrs gene, eis and whiB7 loci displayed novel mutations. The novel mutations displayed by eis and whiB7 loci were found to be associated specifically with the Kanamycin resistance as none of the twenty nine Kanamycin sensitive strains harbor them. The inclusion of novel mutations of eis and whiB7 loci will be useful in improving the specificity of future diagnostics.

Item Type: Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords: Aminoglycosides; MDR-TB; Molecular characterization; XDR-TB
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 04 Oct 2016 05:54
Last Modified: 04 Oct 2016 05:54
URI: http://crdd.osdd.net/open/id/eprint/1916

Actions (login required)

View Item View Item