Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis

Kumar, A and Karthikeyan, Subramanian (2018) Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis. Acta Crystallographica Section F Structural Biology Communications, 74 (3). pp. 166-173. ISSN 2053-230X

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The MSMEG_4306 gene from Mycobacterium smegmatis encodes a protein of unknown function with 242 amino-acid residues that contains a conserved zinc-ribbon domain at its C-terminus. Here, the crystal structure of MSMEG_4306 determined by the single-wavelength anomalous dispersion method using just one zinc ion co-purified with the protein is reported. The crystal structure of MSMEG_4306 shows a coiled-coil helix domain in the N-terminal region and a zinc-ribbon domain in the C-terminal region. A structural similarity search against the Protein Data Bank using MSMEG_4306 as a query revealed two similar structures, namely CT398 from Chlamydia trachomatis and HP0958 from Helicobacter pylori, although they share only ∼15% sequence identity with MSMEG_4306. Based on comparative analysis, it is predicted that MSMEG_4306 may be involved in secretion systems, possibly by interacting with multiple proteins or nucleic acids.

Item Type: Article
Additional Information: Copyright of this article belongs to John Wiley & Sons Inc.
Uncontrolled Keywords: MSMEG_4306; Mycobacterium smegmatis; Rv2229c; X-ray crystallography; coiled-coil helix; zinc SAD; zinc ribbon
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 27 Mar 2018 05:04
Last Modified: 20 Mar 2019 12:33

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