Thauera propionica sp. nov., isolated from downstream sediment sample of the river Ganges, Kanpur, India

Pal, Deepika and Bhardwaj, Ayanka and Sudan, Sarabjeet Kour and Kaur, Navjot and Kumari, Munesh and Bisht, Bhawana and Vyas, Bhawna and Krishnamurthi, Srinivasan and Mayilraj, Shanmugam (2018) Thauera propionica sp. nov., isolated from downstream sediment sample of the river Ganges, Kanpur, India. International Journal of Systematic and Evolutionary Microbiology, 68 (1). pp. 341-346. ISSN 1466-5026

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A Gram-stain-negative, non-endospore-producing, short-rod strain, KNDSS-Mac4T, was isolated from a downstream sediment sample of the river Ganges, Kanpur, India and studied by using the polyphasic taxonomic approach. 16S rRNA gene sequence analysis uncovered that the strain had similarity to species of the genus Thauera and formed a distinct phylogenetic cluster with Thauera humireducens KACC16524T. However, KNDSS-Mac4T showed closest phylogenetic affiliation to Thauera aminoaromatica DSM 14742T with 16S rRNA gene sequence similarity of 98.7 % followed by Thauera phenylacetica DSM 14743T (98.6 %), Thauera chlorobenzoica (98.2 %), T. humireducens KACC16524T (98.2 %), Thauera selenatis ATCC 55363T (98.2 %) and Thauera mechernichensis DSM 12266T (98.0 %). The digital DNA-DNA hybridization and average nucleotide identity values between strain KNDSS-Mac4T and the two most closely related taxa, T. aminoaromatica DSM 14742T and T. phenylacetica DSM 14743T, were 26.0, 26.7 and 84.0, 84.3 % respectively. Major lipids present were phosphatidylglycerol, three unknown aminophospholipids, phosphatidylmethylethanolamine, two unidentified lipids and Q-8 as the only ubiquonone. The major cellular fatty acids present were C16 : 1 ω6c/C16 : 1ω7c and C16 : 0. The DNA G+C content of strain KNDSS-Mac4T was 65.9 %. Based on data from phenotypic tests and the genotypic differences of strain KNDSS-Mac4T from its closest phylogenetic relatives, it is evident that this isolate should be regarded as a new species. It is proposed that strain KNDSS-Mac4T should be classified in the genus Thauera as a novel species, Thauerapropionica sp. nov. The type strain is KNDSS-Mac4T (=KCTC 52820T=VTCC-B-910017T).

Item Type: Article
Additional Information: Copyright of this article belongs to Society for General Microbiology.
Uncontrolled Keywords: 16S rRNA gene; Thauera; dDDH; fatty acid methyl esters
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 27 Mar 2018 05:29
Last Modified: 20 Mar 2019 14:48

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