Mahajan, Sonal and Khairnar, Aasawari and Bishnoi, Ritika and Ramya, T.N.C. (2017) Microbial F-type lectin domains with affinity for blood group antigens. Biochemical and Biophysical Research Communications, 491 (3). pp. 708-713. ISSN 0006291X
Full text not available from this repository. (Request a copy)Abstract
F-type lectins are fucose binding lectins with characteristic fucose binding and calcium binding motifs. Although they occur with a selective distribution in viruses, prokaryotes and eukaryotes, most biochemical studies have focused on vertebrate F-type lectins. Recently, using sensitive bioinformatics search techniques on the non-redundant database, we had identified many microbial F-type lectin domains with diverse domain organizations. We report here the biochemical characterization of F-type lectin domains from Cyanobium sp. PCC 7001, Myxococcus hansupus and Leucothrix mucor. We demonstrate that while all these three microbial F-type lectin domains bind to the blood group H antigen epitope on fucosylated glycans, there are fine differences in their glycan binding specificity. Cyanobium sp. PCC 7001 F-type lectin domain binds exclusively to extended H type-2 motif, Myxococcus hansupus F-type lectin domain binds to B, H type-1 and Lewisb motifs, and Leucothrix mucor F-type lectin domain binds to a wide range of fucosylated glycans, including A, B, H and Lewis antigens. We believe that these microbial lectins will be useful additions to the glycobiologist's toolbox for labeling, isolating and visualizing glycans.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Elsevier. |
| Uncontrolled Keywords: | Blood group antigens; Cyanobium sp. FLD; F-type lectin domain; Fucose; Leucothrix mucor FLD; Myxococcus hansupus FLD |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 28 Mar 2018 05:55 |
| Last Modified: | 28 Mar 2018 05:55 |
| URI: | http://crdd.osdd.net/open/id/eprint/2063 |
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