Pathaniya, Preeti and Sharma, Anurima and Kumar, Binod and Rishi, Praveen and Suri, C Raman (2017) Selective identification of specific aptamers for the detection of non-typhoidal salmonellosis in an apta-impedimetric sensing format. Mcrichimica Acta, 184 (5). pp. 1499-1508.
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The authors report on an aptamer-based method for the detection of S. Typhimurium. The aptamers were identified by using a modified cell-based SELEX method (cell-SELEX) by adopting an alternating negative-positive selection strategy. Each cross-reactive population was used separately, with a focus on generating strain-specific aptamers in order to enhance selectivity. The aptamers were characterized and chemically modified with thiol groups at the 5′ end which enables covalent binding to gold nanoparticles on a screen printed carbon electrode (GNP-SPCE). The aptamer-modified GNP-SPCE was subsequently applied to impedimetric sensing of S. Typhimurium in water and food samples. The response is linear in the 10 to 105 CFU⋅mL−1 concentration range, and the limit of detection is ~10 CFU⋅mL−1. The assay can distinguish S. Typhimurium from S. Typhi and has been validated in water and spiked egg samples.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Springer. |
| Uncontrolled Keywords: | S. Typhimurium; Modified cell-SELEX; Gold nanoparticles; Impedimetry Aptasensor; Microbial pathogen |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 14 Nov 2018 03:16 |
| Last Modified: | 14 Nov 2018 03:17 |
| URI: | http://crdd.osdd.net/open/id/eprint/2126 |
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