Paenibacillus xerothermodurans sp. nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida.

Kaur, Navjot and Seuylemezian, Arman and Patil, Prashant P and Patil, Prabhu and Krishnamurti, Srinivasan and Varelas, Joseph and Smith, David J and Mayilraj, Shanmugam and Vaishampayan, Parag (2018) Paenibacillus xerothermodurans sp. nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida. International journal of systematic and evolutionary microbiology, 68 (10). pp. 3190-3196. ISSN 1466-5034

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Abstract

A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA. Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %). The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7. The polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and one unknown phospholipid. The main fatty acids were iso-C15 : 0 and anteiso-C15 : 0. Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380 to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352 (97.0 %). The other closely related type strains exhibited 16S rRNA gene sequence similarity values below 95.9 %. The draft genome of ATCC 27380 had a size of 4,361,187 bases, with a G+C content of 51.0 %. The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380 and P. rigui JCM 16352 were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively). The average amino acid identity between strain ATCC 27380 and P. rigui JCM 16352 was 68.72 %, which was above the suggested genus level demarcation of 65 %. Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380 represents a novel species in the genus Paenibacillus, for which the name Paenibacillusxerothermodurans sp. nov. (=DSM 520=NRRL NRS-1629=ATCC 27380) is proposed.

Item Type: Article
Additional Information: Copyright of this article belongs to Microbiology Society.
Uncontrolled Keywords: Paenibacillus xerothermodurans; dry heat resistant; novel species
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 19 Mar 2019 09:14
Last Modified: 19 Mar 2019 09:14
URI: http://crdd.osdd.net/open/id/eprint/2154

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