Nagar, Rupa and Rao, Alka (2019) In Vitro Synthesis of Bioactive Glycovariants of Enterocin 96, an Antimicrobial Peptide from Enterococcus faecalis. Methods in molecular biology (Clifton, N.J.), 1954. pp. 279-296. ISSN 1940-6029
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Official URL: https://link.springer.com/protocol/10.1007%2F978-1...
Abstract
Antimicrobial peptides (AMPs) are novel agents for therapeutic application for their inherent broad spectrum of activities against bacteria, fungi, and viruses, as well as anti-inflammatory, anticancerous, and immunomodulatory activities. This chapter presents an enzymatic method to generate glycovariants of one such antimicrobial peptide, namely enterocin 96, using a bacterial protein O- and S-glycosyltransferase, in vitro.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Springer. |
| Uncontrolled Keywords: | Chymotrypsin digestion; Exoglycosidase treatment/Deglycosylation assay; Glycodiversification; High performance liquid chromatography (HPLC); In vitro glycosyltransferase assay; Mass spectrometry |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 18 Mar 2019 16:16 |
| Last Modified: | 18 Mar 2019 16:16 |
| URI: | http://crdd.osdd.net/open/id/eprint/2166 |
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