Sran, Kulwinder Singh and Sundharam, Shiva S and Krishnamurthi, S and Roy Choudhury, Anirban (2019) Production, characterization and bio-emulsifying activity of a novel thermostable exopolysaccharide produced by a marine strain of Rhodobacter johrii CDR-SL 7Cii. International journal of biological macromolecules, 127. pp. 240-249. ISSN 1879-0003
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An exopolysaccharide (EPS) producing bacterial strain was isolated from the surface of marine macroalgae (Padina sp.). Based on polyphasic taxonomy, the strain CDR-SL 7Cii was assigned to the genus Rhodobacter and found to be the closest relative of the species Rhodobacter johrii. The strain was able to produce 6.2 g/l of EPS upon fermentation using R2A medium enriched with 2.5% glucose. FT-IR analysis revealed the presence of hydroxyl, carboxyl and diacyl-ester functional groups in the purified EPS. Further Chromatographic study revealed that R. johrii synthesized a high molecular weight anionic exopolysaccharide composed of glucose, glucuronic acid, rhamnose and galactose in a molar ratio of 3:1.5:0.25:0.25. The 1D and 2D NMR spectroscopy (COSY/HSQC) analysis revealed the presence of 1,6 linked-α-d-Glcp, 1,4 linked-β-d-Glcp, 1,3 linked-β-d-GlcA, 1,3 linked-β-d-Galp, 1,6 linked-β-d-Galf and 3-α-l-Rhmp residues. Moreover, the purified EPS has shown stability towards elevated temperature and also acted as a bio-emulsifier to create a high pH and temperature stable emulsion of hydrocarbon/water indicating its industrial potential. This is the first report of EPS production by a strain of Rhodobacter johrii.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Elsevier Science. |
| Uncontrolled Keywords: | Bio-emulsifier; Exopolysaccharide; Rhodobacter johrii; Structural characterization |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 18 Mar 2019 12:47 |
| Last Modified: | 18 Mar 2019 12:47 |
| URI: | http://crdd.osdd.net/open/id/eprint/2178 |
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