Comparison of ITS and IGS1 regions for strain typing of clinical and non-clinical isolates of Pichia anomala.

Sutar, Rajeshwari and David, J K and Ganesan, K and Ghosh, Anup K and Singhi, Sunit and Chakrabarti, Arunaloke and Bachhawat, Anand K (2004) Comparison of ITS and IGS1 regions for strain typing of clinical and non-clinical isolates of Pichia anomala. Journal of medical microbiology, 53 (Pt 2). pp. 119-23. ISSN 0022-2615

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Official URL: http://jmm.sgmjournals.org/content/53/2/119.long

Abstract

Pichia anomala is an emerging nosocomial pathogen and there is a need for methods that distinguish between different P. anomala strains. In the typing of several clinical as well as non-clinical P. anomala strains, the sequence variation of the internal transcribed spacer (ITS) was found to be inadequate for typing purposes. The intergenic spacer 1 (IGS1) region of the rDNA of several P. anomala strains was therefore investigated in detail. The IGS1 region (which varied from 1213 to 1231 bp in length) was interspersed with repeats and had more variation than the ITS regions. Comparative analysis in cases where analysis by the ITS was ambiguous clearly revealed the IGS1 region to be a more discriminatory tool in the typing of P. anomala strains.

Item Type: Article
Additional Information: Copyright of this article belongs to SGM.
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 06 Jan 2012 14:55
Last Modified: 29 Mar 2012 09:41
URI: http://crdd.osdd.net/open/id/eprint/225

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