Kumar, Sunil and Patil, Prashant P and Singhal, Lipika and Ray, Pallab and Patil, Prabhu B and Gautam, Vikas (2019) Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii isolates reveals the emergence of bla and bla encoding international clones in India. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 75. p. 103986. ISSN 1567-7257
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Acinetobacter baumannii is a nosocomial pathogen increasingly affecting the critically ill patients and represents a major public health challenge. Carbapenem-resistant A. baumannii (CRAB) is found to be associated with International Clones (ICs) and different classes of carbapenemases. The objective of the present study was to investigate the prevalence of carbapenem resistance genes, clonal relationship and genetic structure of clinical isolates of A. baumannii. In the present study, multi-locus sequence typing (MLST) and analysis were carried out using Oxford scheme for 86 clinical isolates of CRAB along with 11 carbapenem sensitive A. baumannii (CSAB) collected over a period of two years (2014-2016) from two tertiary care hospitals of North India. We observed a high prevalence of the bla-like (97.7%) among the CRAB followed by bla (29.1%) and bla-like (3.5%). Forty-seven Sequence Types (STs) were represented by all 97 isolates, out of which, 28 (59.6%) were novel STs that were assigned to 41 isolates. STs 451 (13%), 447 (7%), 195 (6%) and 848 (5%) were the most common STs. The majority of CRAB isolates (44.3%) belonged to the CC92, followed by the CC447 (15.1%), CC109 (9.3%) and CC110 (3.4%), which corresponds to the IC2, 8, 1 and 7 respectively. Phylogenetic and recombination analysis suggested two major and one minor lineage in the population. Further linkage disequilibrium analysis suggested clonal nature of the population as recombination was noticed at a low frequency, which was not enough to split the clonal relationship. The knowledge of genetic structure of CRAB from this study will be invaluable to illustrate epidemiology, surveillance and understanding its global diversity.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Elsevier Science. |
| Uncontrolled Keywords: | Acinetobacter baumannii; And blaNDM-1; Carbapenem resistance; International clones; Multilocus sequence typing; bla(OXA-23)-like |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 14 Aug 2019 10:43 |
| Last Modified: | 16 Aug 2019 14:27 |
| URI: | http://crdd.osdd.net/open/id/eprint/2337 |
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