Singh, Pargat and Bharti, . and Kumar, Rajesh and Bhalla, Vijayender (2019) Gold nanoparticle triggered siloxane formation for polymerization based amplification in enzyme free visual immunoassay. Analytica chimica acta, 1078. pp. 151-160. ISSN 1873-4324
Full text not available from this repository. (Request a copy)Abstract
Herein, we report a new signal amplification scheme for quantitative biochemical analysis based on gold nanoparticle (GNPs) catalyzed polymerization of transparent silane solution to milky white and turbid siloxane. Using immunoassay as a proof of concept, GNP labeled immunoprobe was used to bind captured antigen and catalyse the polymerization reaction allowing sensitive biochemical investigation. The polymerization reaction was optimized for standard 96 well polystyrene microtiter plates and we discovered that sodium lactate acts as an enhancer in the polymerization reaction as it reduces detection time to merely 30 min. The sensing strategy was applied to detection and quantification of Salmonella Typhimurium in water and egg samples and the platform showed excellent visibly quantifiable analytical response up to 100 cells mL. Furthermore, clinical utility and potential of the method was validated by detecting Vi capsular polysaccharide (Vi antigen) responsible for typhoidal Salmonellosis in human serum in sandwich format with a detection limit of 1 ng mL. The method serves as the first report towards nanoparticle triggered polymerization for development of rapid and low cost quantitative biochemical assay.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Elsevier Science. |
| Uncontrolled Keywords: | Gold nanoparticles; Immunoassay; Polymerization; Salmonella typhimurium; Vi capsular polysaccharide |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 14 Aug 2019 10:38 |
| Last Modified: | 14 Aug 2019 10:38 |
| URI: | http://crdd.osdd.net/open/id/eprint/2338 |
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