Roy, Somendu K and Kumari, Neela and Pahwa, Sonika and Agrahari, Udai C and Bhutani, Kamlesh K and Jachak, Sanjay M and Nandanwar, Hemraj (2013) NorA efflux pump inhibitory activity of coumarins from Mesua ferrea. Fitoterapia, 90. pp. 140-50. ISSN 1873-6971
Full text not available from this repository. (Request a copy)Abstract
The purpose of this investigation was to study the modulator and efflux pump inhibitor activity of coumarins isolated from Mesua ferrea against clinical strains as well as NorA-over expressed strain of Staphylococcus aureus 1199B. Seven coumarins were tested for modulator activity using ethidium bromide (EtBr) as a substrate. Compounds 1, 4-7 modulated the MIC of EtBr by ≥ 2 fold against wild type clinical strains of S. aureus 1199 and S. aureus 1199B, whereas compounds 4-7 modulated the MIC of EtBr by ≥ 16 fold against MRSA 831. Compounds 1, 4-7 also reduced the MIC of norfloxacin by ≥ 8 fold against S. aureus 1199B, and 4-6 reduced the MIC of norfloxacin by ≥ 8 fold against MRSA 831 at half of their MICs. Inhibition of EtBr efflux by NorA-overproducing S. aureus 1199B and MRSA 831 confirmed the role of compounds 4-6 as NorA efflux pump inhibitors (EPI). Dose-dependent activity at sub-inhibitory concentration (6.25 μg/mL) suggested that compounds 4 and 5 are promising EPI compared to verapamil against 1199B and MRSA 831 strains.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Elsevier Science. |
| Uncontrolled Keywords: | Clinical strain; Coumarins; Mesua ferrea; NorA-pump inhibitor; Staphylococcus aureus |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 05 Sep 2019 12:03 |
| Last Modified: | 05 Sep 2019 12:03 |
| URI: | http://crdd.osdd.net/open/id/eprint/2452 |
Actions (login required)
 |
View Item |
