Clostridium indicum sp. nov., a novel anaerobic bacterium isolated from the sludge of an industrial effluent.

Gundawar, Krishna and Kumari, Sumeeta and Sharma, Shikha and Grover, Vishakha and Patil, Prabhu B and Korpole, Suresh (2019) Clostridium indicum sp. nov., a novel anaerobic bacterium isolated from the sludge of an industrial effluent. International journal of systematic and evolutionary microbiology, 69 (3). pp. 672-678. ISSN 1466-5034

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A strictly anaerobic bacterial strain, designated as PI-S10-A1B, was isolated from a sludge sample collected from an industrial effluent dump site at Hyderabad, India. Cells stained Gram-positive and contained terminal endospores. Optimal growth was observed at 30 °C and pH 7.0. It showed negative reactions to catalase and oxidase activities. Phylogenetic analysis of the 16S rRNA gene led to strain PI-S10-A1B being assigned to the genus Clostridium. It displayed high sequence similarity to species of cluster XIVa including Clostridium amygdalinumBR-10 (99.84 %), Clostridium saccharolyticum WM1 (98.93 %) and Clostridium indolis DSM 755 (98.31 %). It formed a coherent cluster with members of cluster XIVa. Despite high 16S rRNA gene sequence similarity, strain PI-S10-A1B displayed only 25.3 % identity in DNA-DNA hybridization tests with C. amygdalinum BR-10. A draft genome exhibited low values for average nucleotide identity and in silico DNA-DNA hybridization with strains of cluster XIVa. The DNA G+C content was 42.3 mol%. Major lipids were phosphatidylglycerol and diphosphatidylglycerol, with an abundance of phosphoglycolipids. Further, analysis of the draft genome revealed genomic insights against functional aspects. Considering the phenotypic differences and low genomic identity with phylogenetic relatives, strain PI-S10-A1B is concluded to represent a new species of the genus Clostridium, for which the name Clostridiumindicum sp. nov. is proposed with type strain PI-S10-A1B (=MTCC 12282=DSM 24996=JCM 32788).

Item Type: Article
Additional Information: Copyright of this article belongs to Microbiology Society.
Uncontrolled Keywords: 16S rRNA gene; ANI; C. indicum; anaerobic; genome sequence; polar lipids
Subjects: Q Science > QR Microbiology
Depositing User: Dr. K.P.S.Sengar
Date Deposited: 23 Apr 2020 10:35
Last Modified: 23 Apr 2020 10:35

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