Choukate, Komal and Gupta, Aanchal and Basu, Brohmomoy and Karman, Vrk and Ganguli, Munia and Chaudhuri, Barnali (2020) Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. Journal of structural biology, 209 (2). ISSN 1095-8657
Full text not available from this repository. (Request a copy)Abstract
DivIVA or Wag31, which is an essential pole organizing protein in mycobacteria, can self-assemble at the negatively curved side of the membrane at the growing pole to form a higher order structural scaffold for maintaining cellular morphology and localizing various target proteins for cell-wall biogenesis. The structural organization of polar scaffold formed by polymerization of coiled-coil rich Wag31, which is implicated in the anti-tubercular activities of amino-pyrimidine sulfonamides, remains to be determined. A single-site phosphorylation in Wag31 regulates peptidoglycan biosynthesis in mycobacteria. We report biophysical characterizations of filaments formed by mycobacterial Wag31 using circular dichroism, atomic force microscopy and small angle solution X-ray scattering. Atomic force microscopic images of the wild-type, a phospho-mimetic (T73E) and a phospho-ablative (T73A) form of Wag31 show mostly linear filament formation with occasional curving, kinking and apparent branching. Solution X-ray scattering data indicates that the phospho-mimetic forms of the Wag31 polymers are on average more compact than their phospho-ablative counterparts, which is likely due to the extent of bending/branching. Observed structural features in this first view of Wag31 filaments suggest a basis for higher order Wag31 scaffold formation at the pole.
| Item Type: | Article |
|---|---|
| Additional Information: | The Copyright of this article belongs to Elsevier Science. |
| Uncontrolled Keywords: | Mycobacterial growthBacterial polar growthDivIVAWag31 filamentAFMSAXS |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 31 Dec 2020 06:49 |
| Last Modified: | 31 Dec 2020 06:49 |
| URI: | http://crdd.osdd.net/open/id/eprint/2630 |
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