Maurya, Ranjan Kumar and Alam, M.A. and Nadeem, Ahmed and Prajesh, Rahul and Choudhury, Megha and Bhalla, Vijayender and Agrawal, Ajay (2021) Sensitivity Enhancement of Electrochemical Biosensor for Point of Care (POC) Applications: Vi Antigen Detection as a Case Study. Journal of the Electrochemical Society, 168 (1).
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A novel approach for signal enhancement of electrochemical biosensors by incorporating mechanical vibrations has been developed. We report the electrochemical study of the ferricyanide and ferrocyanide $\left(\,{\left[{\rm{Fe}}{\left({\rm{CN}}\right)}_{6}\right]}^{-3/-4}\right)$ a redox couple, at room temperature (~25 °C), under the effect of mechanical vibrations of different frequencies applied to the sensor, for sensitivity enhancement. The experimental results showed a sensitivity enhancement of ~332% (from $3.125\,{\rm{nA}}\,\mu {{\rm{M}}}^{-1}$ to $13.5\,{\rm{nA}}\,\mu {{\rm{M}}}^{-1}$) at 88.75 Hz of vibration. This novel approach of signal sensitivity enhancement is also validated with antibody immobilization-based Vi antigen detection for typhoid assessment. The sensitivity enhancement up to ~15% is achieved for Vi antigen detection under the mechanical vibration of 120 Hz. The sensor is fabricated using microfabrication technology. The vibration-assisted ultrasensitive biosensing platform is also prototyped as a portable and Internet of Thing (IoT) enabled device, suitable for point-of-care applications. Detailed features of the prototype along with the test results are elaborated in the paper.
| Item Type: | Article |
|---|---|
| Additional Information: | The copyright of this article belongs to IOP Science Publishing. |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 01 Feb 2021 04:18 |
| Last Modified: | 01 Feb 2021 04:18 |
| URI: | http://crdd.osdd.net/open/id/eprint/2648 |
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