Darji, H and Verma , Neha and Lugani, Yogita and Mehrotra, Priyam and Sindhu, DK and Vemuluri, V.R (2021) Polyphasic characterization of and genomic insights into a haloalkali-tolerant Saccharibacillus alkalitolerans sp. nov., that produces three cellulase isozymes and several antimicrobial compounds. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY. ISSN 0003-6072
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A cellulase producing novel bacterial strain VR-M41(T) was isolated from an open-air vegetable and fruit market. Cells are found to be rod-shaped, endospore forming, positive for Gram's stain and negative for catalase, oxidase and urease. Strain VR-M41(T) was halotolerant (upto 8.0% NaCl, w/v), motile and facultative anaerobe. It grew at wide range of pH (6.0-10.0) and temperatures (20-40 degrees C). Strain VR-M41(T) produced three isozymes of Carboxymethylcellulase. The 16S rRNA gene sequence of strain VR-M41(T) was 97.3% similar to both Saccharibacillus kuerlensis DSM 22868(T) and Saccharibacillus sacchari DSM 19268(T), and less than 96.4% with the rest of the valid species of the genus Saccharibacillus. Whole-genome ANI, dDDH and genome phylogenetic tree analysis revealed that strain VR-M41(T) significantly differed from Saccharibacillus kuerlensis DSM 22868(T) and Saccharibacillus sacchari DSM 19268(T) (ANI 79.6-79.7% and dDDH 23.1%). The strain comprised of MK-7 and anteiso-C 15:0 (42.2%) as predominant isoprenoid quinone and fatty acid respectively. Major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The draft genome of strain VR-M41(T) consisted of 5,386,426 base pairs with 5103 annotated genes, out of which 2147 corresponded to hypothetical proteins and 2956 with functional assignments. Pan-genome analysis revealed the presence of 2998 core genes, 828 accessory genes, and 1131 unique genes of Saccharibacillus. Strain VR-M41(T) produced antimicrobials against Staphylococcus aureus, Streptococcus pneumoniae, Micrococcus luteus and Shigella flexneri. Significant phenotypic and genotypic differentiating characteristics from closely related species, indicated that strain VR-M41(T) is a novel species of the genus Saccharibacillus, for which the name Saccharibacillus alkalitolerans sp. nov., is proposed. The type strain is VR-M41(T) (= KCTC 43183(T)=NBRC 114337(T)). Graphic
| Item Type: | Article |
|---|---|
| Additional Information: | The copyright of this article belongs to SPRINGER. |
| Uncontrolled Keywords: | Antimicrobials; Carboxymethylcellulase;Cellulase isozymes;Haloalkali-tolerant;Paenibacillaceae;Saccharibacillus;alkalitolerans |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 28 May 2021 04:02 |
| Last Modified: | 28 May 2021 04:04 |
| URI: | http://crdd.osdd.net/open/id/eprint/2676 |
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