Puri, Anuradhika and Singh, Priyanka and Kumar, Navinder and Kumar, Rajesh and Sharma, Deepak (2021) Tah1, A Key Component of R2TP Complex that Regulates Assembly of snoRNP, is Involved in De Novo Generation and Maintenance of Yeast Prion [URE3]. Journal of molecular biology, 433. ISSN 0022-2836
Full text not available from this repository. (Request a copy)Abstract
The cellular chaperone machinery plays key role in the de novo formation and propagation of yeast prions (infectious protein). Though the role of Hsp70s in the prion maintenance is well studied, how Hsp90 chaperone machinery affects yeast prions remains unclear. In the current study, we examined the role of Hsp90 and its co-chaperones on yeast prions [PSI+] and [URE3]. We show that the overproduction of Hsp90 co-chaperone Tah1, cures [URE3] which is a prion form of native protein Ure2 in yeast. The Hsp90 co-chaperone Tah1 is involved in the assembly of small nucleolar ribonucleoproteins (snoRNP) and chromatin remodelling complexes. We found that Tah1 deletion improves the frequency of de novo appearance of [URE3]. The Tah1 was found to interact with Hsp70. The lack of Tah1 not only represses antagonizing effect of Ssa1 Hsp70 on [URE3] but also improves the prion strength suggesting role of Tah1 in both fibril growth and replication. We show that the N-terminal tetratricopeptide repeat domain of Tah1 is indispensable for [URE3] curing. Tah1 interacts with Ure2, improves its solubility in [URE3] strains, and affects the kinetics of Ure2 fibrillation in vitro. Its inhibitory role on Ure2 fibrillation is proposed to influence [URE3] propagation. The present study shows a novel role of Tah1 in yeast prion propagation, and that Hsp90 not only promotes its role in ribosomal RNA processing but also in the prion maintenance.
| Item Type: | Article |
|---|---|
| Additional Information: | The copyright of this article belongs to Elsevier Science/Academic Press |
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE;LIGAND-DISCRIMINATION;IN-VIVO;CHAPERONE;PROTEIN;HSP90;HSP70;PROPAGATION;PSI;HSP104 |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 22 Jun 2021 05:09 |
| Last Modified: | 22 Jun 2021 05:11 |
| URI: | http://crdd.osdd.net/open/id/eprint/2690 |
Actions (login required)
 |
View Item |
