Kaur, Harsimran and Sasan, Soorya Partap and Yadav, Anita and Martoliya, Yogita and Mondal, Alok K. (2021) Distinct role of HAMP and HAMP-like linker domains in regulating the activity of Hik1p, a hybrid histidine kinase 3 from Magnaporthe oryzae. MOLECULAR GENETICS AND GENOMICS, 296 (5). pp. 1135-1145.
Full text not available from this repository. (Request a copy)Abstract
Nik1 orthologs or group III hybrid histidine kinases (HHK3) represent a unique cytoplasmic osmosensor that act upstream of HOG/p38 MAPK pathway in fungi. It is an important molecular target for developing new antifungal agents against human pathogens. HHK3 orthologs contain a linear array of alternative HAMP and HAMP-like linker domains (poly-HAMP) in the N-terminal region. HAMP domains are quite common in prokaryotic histidine kinases where it mostly functions as signal transducer mediating conformational changes in the kinase domains. In contrast, poly-HAMP in HHK3 acts as a sensor and signal transducer to regulate histidine kinase activity. However, the mechanistic detail of this is poorly understood. Interestingly, recent studies indicate that the poly-HAMP-mediated regulation of the kinase activity varies among the orthologs. Hik1 is an important HHK3 ortholog from fungus Magnaporthe oryzae. In this paper, we aimed to decipher the role HAMP and HAMP-like linker domains in regulating the activity of Hik1p. We show that Hik1p acts as a bona fide osmosensor and negatively regulates the downstream HOG/p38 MAPK pathway in Saccharomyces cerevisiae. Our data suggest a differential role of the HAMP domains in the functionality of Hik1p. Most interestingly, the deletion of individual domains in poly-HAMP resulted in distinct active forms of Hik1p and thereby indicating that the poly-HAMP domain, instead of acting as on-off switch, regulates the histidine kinase activity by transition through multiple conformational states.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright of this article belongs to SPRINGER |
| Uncontrolled Keywords: | Fludioxonil; Fungi; HAMP domain; HOG/p38 pathway; Hybrid histidine kinase 3; Magnaporthe oryzae Hik1; Osmosensor. |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 28 Mar 2022 05:01 |
| Last Modified: | 28 Mar 2022 05:01 |
| URI: | http://crdd.osdd.net/open/id/eprint/2723 |
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