Sundararaman, Aravind and Bansal, Kanika and Sidhic, Jameema and Patil, Prabhu and Halami, Prakash M. (2021) Genome of Bifidobacterium longum NCIM 5672 provides insights into its acid-tolerance mechanism and probiotic properties. ARCHIVES OF MICROBIOLOGY. pp. 6109-6118.

Full text not available from this repository. (Request a copy)

Abstract

Bifidobacterium longum NCIM 5672 is a probiotic strain isolated from the Indian infant feces. The probiotic efficacy of Bifidobacteria is majorly affected by its acid tolerance. This study determined the probiotic properties and acid-tolerance mechanism of B. longum NCIM 5672 using whole-genome sequencing. The genome annotation is carried out using the RAST web server and NCBI PGAAP. The draft genome sequence of this strain, assembled in 63 contigs, consists of 22,46,978 base pairs, 1900 coding sequences and a GC content of 59.6%. The genome annotation revealed that seven candidate genes might be involved in regulating the acid tolerance of B. longum NCIM 5672. Furthermore, the presence of genes associated with immunomodulation and cell adhesion support the probiotic background of the strain. The analysis of candidate acid- tolerance-associated genes revealed three genes, argC, argH, and dapA, may play an essential role in high acid tolerance in B. longum NCIM 5672. The results of RT-qPCR supported this conclusion. Altogether, the results presented here supply an effective way to select acid-resistant strains for the food industry and provide new strategies to enhance this species' industrial applications and health-promoting properties.

Item Type:	Article
Additional Information:	Copyright of this article belongs to SPRINGER
Uncontrolled Keywords:	Bifidobacterium longum; Health benefits; Probiotic marker genes; Whole-genome sequencing.
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	28 Mar 2022 11:18
Last Modified:	28 Mar 2022 11:18
URI:	http://crdd.osdd.net/open/id/eprint/2743

Actions (login required)

View Item