Singh, Shelley Sardul and Akhtar, Md. Naushad and Sharma, Deepika and Mandal, Santi M. and Korpole, Suresh (2021) Characterization of Iturin V, a Novel Antimicrobial Lipopeptide from a Potential Probiotic Strain Lactobacillus sp. M31. PROBIOTICS AND ANTIMICROBIAL PROTEINS. pp. 1766-1779.
Full text not available from this repository. (Request a copy)Abstract
Members of lactic acid bacteria group are known to produce various antimicrobial substances. Cyclic lipopeptides are one such potent class of amphipathic natural biosurfactants that exhibit bactericidal and immunomodulatory properties. In this study, we aimed to investigate antimicrobial and immunomodulatory activities of a lipopeptide secreted by a LAB isolate strain M31 identified as a member of the genus Lactobacillus. The lipopeptide that was purified using a combination of chromatographic techniques and matrix-assisted laser desorption/ionization-time of flight of pure lipopeptide displayed a molecular weight of 1002 Da. MS/MS analysis confirmed the presence of 7 amino acids (Asp-Tyr-Asp-Val-Pro-Asp-Ser) and a C13 beta-hydroxy fatty acid. The amino acid composition assigned lipopeptide to iturin class. However, the replacement of Gln with Val revealed it to represent a novel iturin named as iturin V. Iturin V showed antibacterial activity and did not cause hemolysis or cytotoxicity upto 125 µg/mL. It induced secretion of pro-inflammatory cytokines TNF-alpha and IL-12 in murine dendritic cells. Probiotic features of strain M31 coupled with notable activity of iturin V against species of the genera Pseudomonas and Vibrio suggest that strain M31 has potential application for pathogen intervention treatments in processing of aquatic food products.
| Item Type: | Article |
|---|---|
| Additional Information: | The Copyright of this article belongs to SPRINGER |
| Uncontrolled Keywords: | Fish; Iturin V; Lactobacillus; Lipopeptide; Pro-inflammatory; Pseudomonas; Vibrio. |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 29 Mar 2022 03:49 |
| Last Modified: | 29 Mar 2022 03:49 |
| URI: | http://crdd.osdd.net/open/id/eprint/2753 |
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