Nag, Dhrubajyoti and Farr, Dustin and Raychaudhuri, Saumya and Withey, Jeffrey H. (2022) An adult zebrafish model for adherent-invasive Escherichia coil indicates protection from AIEC infection by probiotic E. coil Nissle. ISCIENCE, 25 (7).
Full text not available from this repository. (Request a copy)Abstract
Adherent-invasive Escherichia coli (AIEC) is an opportunistic pathogen associated with major inflammatory bowel disease, Crohn disease, and ulcerative colitis. Unfavorable conditions push commensal AIEC to induce gut inflammation, sometimes progressing to inflammation-induced colon cancer. Recently, zebrafish have emerged as a useful model to study human intestinal pathogens. Here, a zebrafish model to study AIEC infection was developed. Bath inoculation with AIEC resulted in colonization and tissue disruption in the zebrafish intestine. Gene expression of pro-inflammatory markers including interleukin-1β (IL-1β), tumor necrosis factor alpha (TNFα), interferon-γ (IFNγ), and S100A-10b (akin to human calprotectin) in the zebrafish intestine was significantly induced by AIEC infection. The probiotic E. coli Nissle 1917 (EcN) was tested as a therapeutic and prophylactic against AIEC infection and reduced AIEC colonization, tissue damage, and pro-inflammatory responses in zebrafish. Furthermore, EcN diminished the propionic-acid-augmented hyperinfection of AIEC in zebrafish. Thus, this study shows the efficacy of EcN against AIEC in an AIEC-zebrafish model.
| Item Type: | Article |
|---|---|
| Additional Information: | The copyright of this article belongs to Elsevier |
| Uncontrolled Keywords: | Microbiology; Pathophysiology |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 15 Dec 2022 09:06 |
| Last Modified: | 15 Dec 2022 09:06 |
| URI: | http://crdd.osdd.net/open/id/eprint/3050 |
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