Sarkar, Anindya and Paul, Sathi and Singh, Charandeep and Chowdhury, Nilkanta and Nag, Papri and Das, Swarnava and Kumar, Sahil and Sharma, Anshul and Das, Deepjyoti Kumar and Dutta, Dipak and Thakur, Krishan Gopal and Bagchi, Angshuman and Shriti, Surbhi and Das, Kali P. and Ringe, Rajesh P. and Das, Sampa (2022) A novel plant lectin, NTL-125, interferes with SARS-CoV-2 interaction with hACE2. VIRUS RESEARCH, 315.
Full text not available from this repository. (Request a copy)Abstract
COVID-19 caused by SARS-CoV-2 virus has had profound impact on the world in the past two years. Intense research is going on to find effective drugs to combat the disease. Over the past year several vaccines were approved for immunization. But SARS-CoV-2 being an RNA virus is continuously mutating to generate new variants, some of which develop features of immune escape. This raised serious doubts over the long-term efficacy of the vaccines. We have identified a unique mannose binding plant lectin from Narcissus tazetta bulb, NTL-125, which effectively inhibits SARS-CoV-2 replication in Vero-E6 cell line. In silico docking studies revealed that NTL-125 has strong affinity to viral Spike RBD protein, preventing it from attaching to hACE2 receptor, the gateway to cellular entry. Binding analyses revealed that all the mutant variants of Spike protein also have stronger affinity for NTL-125 than hACE2. The unique α-helical tail of NTL-125 plays most important role in binding to RBD of Spike. NTL-125 also interacts effectively with some glycan moieties of S-protein in addition to amino acid residues adding to the binding strength. Thus, NTL-125 is a highly potential antiviral compound of natural origin against SARS-CoV-2 and may serve as an important therapeutic for management of COVID-19.
| Item Type: | Article |
|---|---|
| Additional Information: | The copyroght of this article belongs to Elsevier |
| Uncontrolled Keywords: | Molecular docking; Mutant variants, Narcissus tazetta lectin; SARS-CoV-2; Spike Protein |
| Subjects: | P Language and Literature > PZ Childrens literature Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 15 Dec 2022 09:36 |
| Last Modified: | 15 Dec 2022 09:36 |
| URI: | http://crdd.osdd.net/open/id/eprint/3060 |
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