Chemotaxis and biodegradation of 3-methyl- 4-nitrophenol by Ralstonia sp. SJ98.

Bhushan, B and Samanta, S K and Chauhan, Archana and Chakraborti, Asit K and Jain, R K (2000) Chemotaxis and biodegradation of 3-methyl- 4-nitrophenol by Ralstonia sp. SJ98. Biochemical and biophysical research communications, 275 (1). pp. 129-33. ISSN 0006-291X

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Abstract

3-Methyl-4-nitrophenol is one of the major breakdown products of fenitrothion [O,O-dimethyl O-(3-methyl-4-nitrophenyl) thiophosphate], a recalcitrant organophosphate insecticide used in agriculture. Being the non-polar methylated aromatic compound, 3-methyl-4-nitrophenol is highly toxic and, therefore, a complete degradation of this compound is important for environmental decontamination/bioremediation purposes. A gram negative, motile Ralstonia sp. SJ98 was isolated by selective screening from a soil sample contaminated with pesticides. The microorganism was capable of utilizing 3-methyl-4-nitrophenol as the sole source of carbon and energy. Thin layer chromatography (TLC), gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), and high performance liquid chromatography (HPLC) were performed to determine the possible intermediates in the degradative pathway of this compound. Taken together, catechol was found to be one of the major intermediate of the pathway. Furthermore, the chemotactic behavior of Ralstonia sp. SJ98 towards 3-methyl-4-nitrophenol was tested using three different methods i.e., drop assay, swarm plate assay and capillary assay, which were found to be positive towards this compound. This is the first report clearly indicating the involvement of a microorganism in the chemotaxis and biodegradation of methyl-4-nitrophenol and formation of catechol as an intermediate in the degradative pathway.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Uncontrolled Keywords:	Ralstonia sp. SJ98; 3-methyl-4-nitrophenol degradation; catechol formation; chemotaxis
Subjects:	Q Science > QD Chemistry
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	04 Jan 2012 14:59
Last Modified:	07 Jan 2015 10:27
URI:	http://crdd.osdd.net/open/id/eprint/309

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