Verma, Himanshu and Mihooliya, Kanti N. and Nandal, Jitender and Sahoo, Debendra K. (2024) Studies on a new antimicrobial peptide from Vibrio proteolyticus MT110. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 54 (2). pp. 193-206.
Full text not available from this repository. (Request a copy)Abstract
The marine environment is known for its vast diversity of the microbial population; however, less explored for bioactive compounds. In this study, an AMP produced by a new marine isolate, Vibrio proteolyticus MT110, showed broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria. The AMP was purified to homogeneity using ethyl acetate extraction followed by RP-HPLC, and LC-MS analysis showed its molecular weight as 980 Da. The MIC of AMP (peptide-MT110) was obtained in the 7.81-31.25 µg/mL range against different indicator strains. Peptide-MT110 showed stability of its antimicrobial activity at 15-121 °C and pH 4-10 and in the presence of various hydrolytic enzymes. The peaks at 1536 cm-1 and 1712 cm-1 wavenumbers in FTIR spectra confirmed the peptidic nature of AMP, and its amino acid analysis confirmed the presence of tyrosine and isoleucine. The antibacterial activity of peptide-MT110 is confirmed by PI assay and TEM. The optimization of peptide-MT110 production using statistical methods resulted in a 2.64-fold higher production. The physicochemical properties and stability in wide pH and temperature ranges showed the potential of peptide-MT110 for its development as a drug candidate. This is believed to be the first report on an AMP from Vibrio proteolyticus.
| Item Type: | Article |
|---|---|
| Additional Information: | The copyright of this article belongs to TAYLOR AND FRANCIS |
| Uncontrolled Keywords: | Antimicrobial peptide; MIC; Vibrio proteolyticus; amino acid analysis; response surface methodology. |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 24 Jun 2024 02:36 |
| Last Modified: | 24 Jun 2024 02:36 |
| URI: | http://crdd.osdd.net/open/id/eprint/3107 |
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