Bhardwaj , Mahipal and Kamble , Pranay and Mundhe , Priyanka and Jindal, Monika and Thakur, Payal and Bajaj, Priyanka (2023) Multifaceted personality and roles of heme enzymes in industrial biotechnology. 3 Biotech, 13 (389). ISSN 2190-572X
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Heme enzymes are the most prominent category of iron-containing metalloenzymes with the capability of catalyzing an astonishingly wide range of reactions like epoxidation, hydroxylation, demethylation, desaturation, reduction, sulfoxidation, and decarboxylation. Various enzymes in this category are P450s, heme peroxidases, catalases, myoglobin, cytochrome C, and others. Besides this, the natural promiscuity and amenability of these enzymes to protein engineering and evolution have also added several non-native reactions such as C-H, N-H, S-H insertions, cyclopropanation, and other industrially important reactions to their capabilities. Surprisingly, all of these reactions and their wide substrate scopes are attributed to changes in the active site scaffold of different heme enzymes as the center of all enzymes is constituted by a porphyrin ring containing iron. Multiple prominent research groups across the world, including 2018, Nobel Laureate Frances Arnold's group, have shown keen interest in engineering and evolving these enzymes for utilizing their industrial potential. Besides engineering the active site, researchers have also explored the possibility of these enzymes catalyzing non-native reactions by replacing the center porphyrin ring with other cofactors or by changing the iron in the porphyrin ring with other metal ions along with engineering the active site and thereby creating novel artificial metalloenzymes. Thus, in this mini-review from our group, for the first time, we are trying to catalog various activities catalyzed by heme enzymes and their engineered variants and their active usage in various industries along with shedding light on their potential for use in various applications in the future.
| Item Type: | Article |
|---|---|
| Additional Information: | The copyright of this article belongs to Springer Link |
| Uncontrolled Keywords: | Artificial metalloenzymes; Biocatalyst; Catalase; Green chemistry; Heme; Industrial biotechnology; Myoglobin; P450; Peroxidase |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 16 Jul 2024 02:22 |
| Last Modified: | 16 Jul 2024 02:22 |
| URI: | http://crdd.osdd.net/open/id/eprint/3165 |
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