Kaur, Amandeep and Rana, Rekha and Bansal, Kanika and Patel, Hitendra K. and Sonti, Ramesh V. and Patil, Prabhu B (2023) Insights into the Diversity of Transcription Activator-Like Effectors in Indian Pathotype Strains of Xanthomonas oryzae pv. oryzae. Phytopathology, 113 (6). pp. 953-959. ISSN 0031-949X
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Xanthomonas oryzae pv. oryzae (Xoo) is a major rice pathogen, and its genome harbors extensive inter-strain and inter-lineage variations. The emergence of highly virulent pathotypes of Xoo that can overcome major resistance (R) genes deployed in rice breeding programs is a grave threat to rice cultivation. The present study reports on a long-read Oxford nanopore-based complete genomic investigation of Xoo isolates from 11 pathotypes that are reported based on their reaction toward 10 R genes. The investigation revealed remarkable variation in the genome structure in the strains belonging to different pathotypes. Furthermore, transcription activator-like effector (TALE) proteins secreted by the type III secretion system display marked variation in content, genomic location, classes, and DNA-binding domain. We also found the association of tal genes in the vicinity of regions with genome structural variations. Furthermore, in silico analysis of the genome-wide rice targets of TALEs allowed us to understand the emergence of pathotypes compatible with major R genes. Long-read, cost-effective sequencing technologies such as nanopore can be a game changer in the surveillance of major and emerging pathotypes. The resource and findings will be invaluable in the management of Xoo and in appropriate deployment of R genes in rice breeding programs.
| Item Type: | Article |
|---|---|
| Additional Information: | The copyright of this article belongs to The American Phytopathology |
| Uncontrolled Keywords: | Oxford Nanopore; Xanthomonas oryzae pv. oryzae; pathotype; resistance (R) genes; transcription activator-like effectors |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 16 Jul 2024 05:04 |
| Last Modified: | 16 Jul 2024 05:04 |
| URI: | http://crdd.osdd.net/open/id/eprint/3179 |
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