Choudhury, Jagrity and Chaudhuri, Barnali N (2025) Tubules, rods, and spirals: Diverse modes of SepF-FtsZ assembling. Cytoskeleton (Hoboken), 82 (7). pp. 432-443.
Full text not available from this repository.Abstract
Z-ring formation by FtsZ, the master assembler of the divisome, is a key step in bacterial cell division. Membrane anchoring of the Z-ring requires the assistance of dedicated Z-ring binding proteins, such as SepF and FtsA. SepF participates in bundling and membrane anchoring of FtsZ in gram-positive bacteria. We report in vitro biophysical studies of the interactions between FtsZ and a cytoplasmic component of cognate SepF from three different bacteria: Mycobacterium tuberculosis, Staphylococcus aureus, and Enterococcus gallinarum. While the cytosolic domain of SepF from M. tuberculosis is primarily a dimer, those from S. aureus and E. gallinarum polymerize to form ring-like structures. Mycobacterial SepF helps in the bundling of FtsZ filaments to form thick filaments and large spirals. On the other hand, ring-forming SepF from the Firmicutes bundle FtsZ into tubules. Our results suggest that the oligomeric form of SepF directs how it bundles FtsZ filaments.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FtsZ bundling; SepF; SepF-FtsZ interaction; bacterial cell division; bacterial cytoskeleton |
| Depositing User: | Dr. K.P.S.Sengar |
| Date Deposited: | 02 Feb 2026 07:23 |
| Last Modified: | 02 Feb 2026 07:23 |
| URI: | http://crdd.osdd.net/open/id/eprint/3211 |
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