Localization of DnaK and GroEL in Vibrio cholerae.

Jyot, J and Gautam, J K and Raje, Manoj and Ghosh, A (1999) Localization of DnaK and GroEL in Vibrio cholerae. FEMS microbiology letters, 172 (2). pp. 165-71. ISSN 0378-1097

PDF ghosh99.pdf - Published Version Restricted to Registered users only Download (349Kb) | Request a copy

Abstract

Though the GroEL and DnaK heat shock proteins are well characterized in prokaryotes, only scanty and controversial information exist about their cellular localization. In the present study, the localization of the heat shock proteins DnaK and GroEL in normal and heat shocked cells of Vibrio cholerae, was investigated both by immunogold labeling of ultrathin sections and biochemical methods. Much of the DnaK was found to be localized at the inner membrane in unstressed cells, most probably at the Bayer's adhesion sites. Data suggested that upon heat shock, the DnaK associated with the membrane continued to remain there, but the newly synthesized DnaK appeared mostly in the cytoplasm. GroEL in both stressed and unstressed cells was found mainly in the cytoplasm.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Science
Uncontrolled Keywords:	Vibrio cholerae; DnaK; GroEL; Localization
Subjects:	Q Science > QR Microbiology
Depositing User:	Dr. K.P.S.Sengar
Date Deposited:	03 Jan 2012 16:50
Last Modified:	07 Jan 2015 05:58
URI:	http://crdd.osdd.net/open/id/eprint/345

Actions (login required)

View Item