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@ARTICLE{Mittal2026-mq,
  title     = "Pseudomonas Pichavaramensis sp. nov., Isolated from Brackish
               Water of Pichavaram Mangrove forest, Tamil Nadu",
  author    = "Mittal, Sumit and Kushwaha, Hariom and {Nandini} and Choksket,
               Stanzin and Prabhu, Devachandana C and Pinnaka, Anil Kumar and
               Korpole, Suresh",
  abstract  = "A cream-white bacterial colony designated SH1-B was isolated on
               Tryptone Soya Agar (TSA) plated with a brackish water sample
               collected from the Pichavaram mangrove forest. The strain SH1-B
               was Gram-stain negative, rod-shaped, motile, non-spore-forming
               aerobic bacterium. Catalase and oxidase activities were
               positive. Cells grew between 15 and 37 °C and pH 5.0--9.0. The
               nucleotide sequence of 16 S rRNA gene displayed high similarity
               with species of the genus Pseudomonas, including P. khazarica
               TBZ2T (98.5\%), P. yangonensis MY50T (98.0\%) and P. oleovorans
               subsp. oleovorans DSM1045T (98.0\%). Genome sequence analysis of
               strain SH1-B showed 74.2--87.6\% average nucleotide identity
               (ANI) with the type strains of the closest species.
               Additionally, digital DNA-DNA hybridization (dDDH) analysis
               revealed low relatedness, with a maximum value of 34.9\%
               observed for its closest phylogenetic relative, P. khazarica
               TBZ2T. The G + C content of the genomic DNA was 62.2\%. The
               major polar lipids were phosphatidylethanolamine (PE),
               phosphatidylglycerol (PG) and an unidentified phospholipid
               (PL1). The predominant cellular fatty acids of strain SH1-B
               included C18:1$\omega$7c, C16:0, C12:0, summed feature 3
               (C16:1$\omega$6c/C16:1$\omega$7c). Considering the phenotypic
               differences and low genomic identity compared to the closest
               species, strain SH1-B was identified as a novel species within
               the genus Pseudomonas, for which the name Pseudomonas
               pichavaramensis sp. nov. is proposed. The type strain is SH1-BT
               (= MTCC 13546T = JCM 37389T = ICMP 25819T).",
  journal   = "Curr. Microbiol.",
  publisher = "Springer Science and Business Media LLC",
  volume    =  83,
  number    =  4,
  pages     = "176",
  month     =  feb,
  year      =  2026,
  copyright = "https://www.springernature.com/gp/researchers/text-and-data-mining",
  language  = "en"
}
